Acute myocardial infarction (AMI) causes heart muscle damage and the release of troponin complex that can be detected in patients' blood by the measurement of its components. Cardiac troponin I (cTnI) and cardiac troponin T (cTnT). Both proteins are widely used nowadays as the guideline recommended markers of acute myocardial infarction and myocardial injury associated with postoperative myocardium trauma, chemotherapy cardiotoxicity and many other diseases related to cardiac muscle injury.

HyTest offers recombinant troponin ITC complex (Cat.# 8ITCR) constructed from recombinant cTnI, cTnT and TnC. Resulting complex is separated from free proteins and dimeric complex. The recombinant troponin ITC complex is similar to the native troponin complex isolated from cardiac tissue (Fig. 1) confirmed by our in-house Tcom8 (Cat.# 4TC2) - 7E7 (Cat.# 4T19) assay. The new recombinant troponin complex can be used as a standard in immunoassays for the detection of cTnI and cTnT as a calibrator.

Figure 1. Comparison of recombinant cTn ITC and native cTn ITC complex using in-house assay. The Tcom8 (Cat.# 4TC2) - 7E7 (Cat.# 4T19) assay detects cardiac troponin complex only if all three troponin isoforms are bound together.

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Cat.# 8ITCR: Recombinant human cardiac troponin ITC-complex