Canine, Feline and Equine SAA Antibodies and Antigens

We provide eight well-characterized anti-SAA MAbs for the detection of feline, canine and equine SAA. Isotypes and specificities of the antibodies are shown in Table 1. Six MAbs recognize feline SAA in direct ELISA and Western Blotting. All MAbs recognize canine and equine SAA in direct ELISA and Western Blotting.

Serum amyloid A (SAA) is a major acute phase protein in many species including dogs (canine), cats (feline) and horses (equine).


Serum amyloid A (SAA) is a major acute phase protein in many species including dogs (canine), cats (feline) and horses (equine). SAA is a sensitive marker of inflammation and tissue damage.

We provide eight well-characterized anti-SAA MAbs for the detection of feline, canine and equine SAA. Isotypes and specificities of the antibodies are shown in Table 1. Six MAbs recognize feline SAA in direct ELISA and Western Blotting. All MAbs recognize canine and equine SAA in direct ELISA and Western Blotting.

We also provide recombinant canine, feline and equine SAA proteins.

Table 1. Characteristics and specificities of anti-SAA MAbs.


Antibody pair recommendations

The most sensitive capture-detection pairs are given in the table below. These recommendations are based on the results obtained using our in-house time-resolved fluorescence immunoassay.

Sandwich immunoassays for feline, canine and equine SAA

All 8 antibodies recognize SAA from dog and horse serum and 6 antibodies recognize SAA from cat serum. For the development of an immunoassay that allow the detection of feline SAA, we recommend the antibody combinations SAA19-VSA34 and SAA21-VSA34. These two combinations could also be used for detecting canine and equine SAA. Figure 1 shows the detection of SAA in serum samples obtained from cats (A), dogs (B) and horses (C) using the antibodies SAA19 and VSA34 for capture and detection respectively.

Figure 1. Comparison of SAA immunoreactivity in serum samples obtained from healthy and diseased cats (A), dogs (B) and horses (C) detected by using the MAb combination SAA19-VSA34. Serum samples were either diluted 800-fold (cat and horse serum) or 500-fold (dog serum). SAA immunoreactivity in serum samples obtained from diseased animals was considerably higher as compared to SAA immunoreactivity in normal serum samples.

A dilution curve of recombinant feline SAA for the MAb combination SAA19-VSA34 is provided in Figure 2.

Figure 2. Dilution curve of recombinant feline SAA for the MAb combination SAA19-VSA34.

Antibody pairs VSA2-VSA38, VSA2-VSA31 and VSA38-VSA43 are recommended for the development of sandwich immunoassays that enable the detection of canine and equine SAA with high sensitivity and specificity. Dilution curves of recombinant canine (A) and equine (B) SAA for the MAb combination VSA2-VSA38 is provided in Figure 3.

Figure 3. Dilution curves of recombinant canine (A) and equine (B) SAA for the MAb combination VSA2-VSA38.

 


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