Neurofilament light (NfL) TechNotes

Neurofilaments are the main cytoskeletal structure proteins in neurons. They are approximately 10 nm in diameter, which makes them thicker than actin and thinner than myosin. Therefore, they are classed as intermediate filaments (IFs) along with filaments built of keratins, nuclear lamins, and other members of the IF protein family. Neurofilaments comprise four different subunits, the stoichiometry of which varies depending on the maturity of the neuron. Three of these subunits, NfL (light), NfM (middle), and NfH (heavy), are type IV IF proteins and they are always present. Meanwhile, the fourth subunit is either α-internexin (also type IV) or peripherin (type III), for the central and peripheral nervous systems, respectively. The NfM and NfH, which have long and highly charged C-terminal domains, are mostly found on the outside of the filaments whereas NfL, along with α-internexin or peripherin, forms the filament backbone. Thus, NfL plays a crucial role for the neurofilament polymerization process as well as for axonal structure maintenance.

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Cat# 4NF3:Neurofilament light (NfL), human, antibody